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  • 名稱:鑷子電極

  • 型號(hào):Tweezertrodes
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用于體內(nèi)**/基因/蛋白質(zhì)傳遞及基因**電極
詳情介紹:

Tweezertrodes電極是一種可復(fù)用的小鉗狀電極,主要用于體內(nèi)的**或基因的傳遞。電極是一個(gè)標(biāo)準(zhǔn)的11.5厘米的小鉗子,其尖部為嵌入的不銹鋼圓盤狀電極,兩盤的間隙可調(diào)至2厘米,并有一個(gè)陽(yáng)極指示器。

Tweezertrodes電極有兩種規(guī)格,45-0118為直徑:7mm的電極盤,45-0119為直徑:10mm的電極盤

當(dāng)把感興趣的分子注射到體內(nèi)后,用電極盤夾住相關(guān)組織,然后給出電脈沖,使孔道開始形成,將分子整合到直接與電極盤接觸的組織細(xì)胞內(nèi)。
Tweezertrodes are reusable, tweezer-style in vivo electrodes for drug or gene delivery in animals. Tweezertrodes consist of a standard 11.5 cm tweezer that has been modified with stainless steel circular or disk electrodes at the tip. The gap between the electrode disks may be adjusted from under 1 mm to over 2 cm. Tweezertrodes come in two models. Model 520 has an electrode disk diameter of 7 mm and Model 522 has a diameter of 10 mm. Tweezertrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. These electrodes are connected to a pulse generator with the Model 524 Connection Cable, and are compatible with most BTX Electroporation System
Applications

Tweezertrodes may be used for many in vivo applications, including gene and drug delivery. Following localized or systemic injection of the molecule of interest, the Tweezertrode electrode disks are used to grasp the tissue of interest. An electroporation pulse is then given, initiating pore formation and incorporation of the molecule into the cells of the tissue in direct contact with the electrode disks. Suzuki et al. used Tweezertrodes to deliver a Green Fluorescent Protein (GFP) gene into rat liver. The authors demonstrated successful gene transfer and expression in situ.1,2 Saito and Nakatsuji used a similar electrode to introduce plasmid DNA during ex-utero and in-utero electroporation.7 Sato et al. used the Tweezertrode with the T820 to introduce EGFP expressing plasmid in mouse testis.8 Tweezertrodes complement BTX Genetrode Models 508, 510, Caliper Electrode Models 384 and 384L, 2-Needle Array Models 530 and 532 as the newest addition to our in vivo electrode line. The Tweezertrodes can be used to reproduce applications requiring the caliper electrodes, including gene therapy, transdermal drug delivery and electroporation therapy.3,4,5,6

Technical Specifications


Standard Capabilities
Voltage Range: 0V - 200V (Do not use AC current)
Pulse Length Range: 1 µsec - 200 msec
Physical Characteristics
Model 520
Tweezertrode Length: 11.5 cm
Electrode Diameter: 7 mm
Electrode Material: Stainless steel
Model 522
Tweezertrode Length: 11.5 cm
Electrode Diameter: 10 mm
Electrode Material: Stainless steel
Generator Compatibility ECM T820, 630, 830, 2001

Ordering Information

System Model Available Configurations
520 Tweezertrode 520
Tweezer Electrode with stainless steel plated 7 mm diameter electrode disk for in vivo applications

523 Tweezertrode 523
Tweezer Electrode with stainless steel plated 10mm diameter electrode disk for in vivo applications

Accessory Model Description
524 Tweezertrode Connection Cable

References
Suzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivo Electroporation, FEBS Letters, 425: 436-440 (1998)
BTX Electroporation Protocol PR0363 (1998)
Zhang et al., In vivo transdermal delivery of large molecules by pressure-mediated electroincorporation and electroporation: a novel method for drug and gene delivery, Bioelectrochemistry and Bioenergetics, 42: 283-292 (1997)
Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotid artery by electroporation, Third U.S. / Japan Symposium on Drug Delivery Systems (Abstr.), 38 (1995)
Dev, Killing cancer cells with a combination of pulsed electric fields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)
BTX Electroporation Therapy Protocols ECT001-ECT005 (1995)
Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001)
Sato et al., Molecular Reproduction and Development, 61: 49-56 (2002)

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