體外胚胎基因傳遞：如可進行小鼠體外胚胎基因傳遞（IVEGD）
體內(nèi)基因傳遞：可用于體內(nèi)，如將基因轉(zhuǎn)入完整神經(jīng)組織
BTX Genepaddles 是一種非插入式槳形可重復(fù)使用的電極。專為體內(nèi)和體外應(yīng)用，如鼠胚胎基因轉(zhuǎn)移等而設(shè)計，適用于多個領(lǐng)域。這些電極頭都是鍍金的，有兩種款式，每一種款式都包括了一對電極。在注射目的分子后，將電極置于胚胎前部和后部，使用BTX電穿孔系統(tǒng)施加電脈沖，Genepaddles可以用電極夾持器在組織內(nèi)以一種預(yù)設(shè)的間隙平行放置。
Genepaddles有兩種尺寸：3 x 5mm和5 x 7mm槳片。 可以適用于電穿孔靶區(qū)大小不同的需要。這些電極連接到45-0201方型柱式電纜，又通過45-0210香蕉形共軸電纜與脈沖發(fā)生器相接。
The BTX Genepaddles are paddle-style reusable electrodes suitable for a variety of applications. Genepaddles come in two models, each model consisting of a pair of electrodes. The Model 542 Genepaddles feature rectangular 3 x 5mm paddles, while the Model 543 Genepaddles feature rectangular 5 x 7mm paddles. The models vary to suit the size of the target electroporation area. These electrodes are connected to a Model 465 Square-Post Connection Cable and interfaced with a pulse generator via a Model 5343 Coaxial-banana Connection cable. The Genepaddles are positioned in parallel at a predetermined gap within a tissue using the Model 515 Genetrode Holder. Genepaddles may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. Properly maintained Genepaddles have a life span of approximately 1500+ pulses, and are compatible with most BTX Electroporation Systems.
Applications
In Vitro Embryo Gene Delivery
Genepaddles were designed for In Vitro Embryo Gene Delivery (IVEGD) in mouse embryo. The electrodes are placed anterior and posterior to the embryo following injection of the molecule of interest, and an electroporation pulse is delivered using a BTX Electroporation System, such as the ECM® 830, 2001, 630, 600, and T820. The electric field introduced by the Genepaddles causes transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells. Itasaki1 used a 3 x 5mm Genepaddle-style electrode with a BTX T820 to deliver DNA to mouse embryos in vitro. His success demonstrates the potential of electroporation to transfect different types of tissues or embryos besides the classical avian embryo.
In Vivo Gene Delivery
Paddle-style electrodes have also been used for in vivo applications. Miyasaka2 used an electrode of this style with a BTX T820 to deliver DNA in vivo into the cerebral cortical anlage of E15 rat embryos. He demonstrated high-efficiency gene transfer into intact neural tissue using electroporation. Electroporation has the benefits of being less labor-intensive and more economical than competitive methods for in vivo gene transfer, such as viral methods and biolistics. Electroporation also produces greater viability and efficiency than competitive methods for in vivo gene transfer.
Technical Specifications
Standard Capabilities
Voltage Range: 0 - 200 V DC
Pulse Length Range: 1µsec - 99msec
Pulse Number Range: 1-99 (depending on voltage and pulse length settings)
Physical Characteristics
Model 542 Model 543
Paddle Dimensions: 3 x 5mm 5 x 7mm
Paddle Configuration: Rectangular Rectangular
Paddle Thickness: 1mm 1mm
Gold Plating Thickness: 0.04mm 0.04mm
All Genepaddle electrodes are gold plated, noninvasive electrodes
Generator Compatibility: ECM® 830, 2001, 630, 600, T820
Genetrode Holder Inter-electrode Gaps: 1-10mm
Ordering Information
Genepaddles Description Part Number
542 3 X 5 mm Genepaddles 10-002892-01
543 5 X 7 mm Genepaddles 10-002896-01
Accessories Description Part Number
515 Genetrode Holder 10-002560-01
465 Square-Post Cable 06-700044-01
5343 Banana-Coaxial Connection Cable 06-700042-01
4001 Enhancer 400 w/ Computer and Printer 01-001483-01
References
Itasaki et al., ''Shocking'' developments in chick embryology: electroporation and in ovo gene expression, Nature Cell Biology, 1: E203-E207 (1999) BTX Reference ID#4699
Miyasaka et al., Foreign gene expression in an organotypic culture of cortical anlage after in vivo electroporation, NeuroReport 10: 2319-2323 (1999) BTX Reference ID#464