BTX Caliper 電極是一種體內(nèi)可復(fù)用的卡鉗狀測(cè)徑器電極，有多種應(yīng)用，如在體內(nèi)進(jìn)行基因和**的傳遞，也可經(jīng)皮膚應(yīng)用及在完整的植物上應(yīng)用。該電極由一個(gè)測(cè)徑器和一對(duì)末端電極組成，共有兩種類型：45-0101型的末端為一對(duì)1x1cm黃銅制末端電極，適用于小動(dòng)物，45-0102 型的末端為一對(duì)1.5x1.5cm或2x2cm的不銹鋼制末端電極，適用于較大的區(qū)域表面，兩電極間的距離可以調(diào)節(jié)。
當(dāng)把感興趣的分子注射到體內(nèi)以后，用電極板夾住相關(guān)目標(biāo)組織，然后給出電脈沖，電場(chǎng)通過(guò)電極在組織細(xì)胞間形成暫時(shí)的孔道，使分子進(jìn)入到與電極接觸的組織細(xì)胞內(nèi)。
The BTX Caliper Electrodes are in vivo style re-usable electrodes that are used for a variety of applications. Caliper Electrodes come in two models, with each model consisting of a caliper and a pair of end plates. Model 384 are used for smaller animals with a smaller surface area, while model 384L is used to target slightly larger surface areas. The end plates of the Caliper Electrode may be adjusted by using the black roller mounted on the caliper. Caliper Electrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. Properly maintained Caliper Electrodes have an unlimited life, and are compatible with most BTX Electroporation Systems.
Applications
Caliper Electrodes may be used for many electroporation applications, including drug and gene delivery. The electrodes span the target area following injection of the molecule of interest, and electroporation pulses are delivered using a BTX Electroporation System, such as the ECM 600, ECM 630, ECM 830, ECM 2001, or T820. The electric field introduced by the Caliper Electrodes cause transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells. Prud''Homme et al. used the Caliper electrodes to deliver plasmid DNA to skin for gene therapy studies on diabetes.1 Mir et al. used the Caliper Electrode for in vivo skeletal muscle work2 as well as subcutaneous tumor electrochemotherapy applications3. Caliper Electrodes can potentially be used for intact plant work as well.4
Caliper Electrodes complement BTX Tweezertrodes Model 520, 522; BTX Genetrodes Model 508(S) and 510(S); and BTX 2 Needle Array Electrodes Model 530, 531, 532, 533 as part of the BTX in vivo electrode line. Caliper Electrodes can be used to reproduce applications requiring Tweezertrodes (transdermal) and Genetrodes/2-Needle.
Technical Specifications
Standard Capabilities
Voltage Range: 0-500V DC(depending on electrode gap)
Pulse Length Range: 1mses-10msec
Pulse Number Range: 1-99 (depending on voltage)
Physical Characteristics
Model 384 Electrode Dimensions: 1.0cm x 1.0cm
Model 384L Electrode Dimensions: 2.0cm x 2.0cm (1.5cm x 1.5cm spare pair included)
Electrode Material: Stainless Steel
Generator Compatibility: ECM 830, 600, 630, 2001, T820
Electrode gap: 0.1-13cm
Ordering Information
System Model Available Configurations
384 Caliper Electrodes, 1.0cm x 1.0cm Brass Plates
384L Caliper Electrodes, 2.0cm x 2.0cm Stainless Steel Plates (1.5cm x 1.5cm spare pair included)